The cases were led by progressive consumer watchdog Public Citizen, the American Public Health Association and National Security Counselors, a public interest law firm. They seek to block co-heads Elon Musk and Vivek Ramaswamy from moving forward or coordinating with the new administration.
Musk and Ramaswamy have pledged ambitious plans to cut $2 trillion in government spending and restructure federal agencies, though Ramaswamy is also planning to run for Ohio governor.
The three cases were some of the first lawsuits filed against the new Trump administration after the president took the oath of office in the Capitol.
Trump has vowed to sign dozens of executive orders later in the day, which could also quickly spark a wave of litigation.